Structured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy

TitleStructured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy
Publication TypeJournal Article
Year of Publication2017
AuthorsChowdhury, S, Eldridge, WJ, Wax, A, Izatt, JA
JournalBiomedical Optics Express
Volume8
Issue5
Start Page2496
Pagination2496 - 2496
Date Published05/2017
Abstract

Sub-diffraction resolution imaging has played a pivotal role in biological research by visualizing key, but previously unresolvable, sub-cellular structures. Unfortunately, applications of far-field sub-diffraction resolution are currently divided between fluorescent and coherent-diffraction regimes, and a multimodal sub-diffraction technique that bridges this gap has not yet been demonstrated. Here we report that structured illumination (SI) allows multimodal sub-diffraction imaging of both coherent quantitative-phase (QP) and fluorescence. Due to SI's conventionally fluorescent applications, we first demonstrate the principle of SI-enabled three-dimensional (3D) QP sub-diffraction imaging with calibration microspheres. Image analysis confirmed enhanced lateral and axial resolutions over diffraction-limited QP imaging, and established striking parallels between coherent SI and conventional optical diffraction tomography. We next introduce an optical system utilizing SI to achieve 3D sub-diffraction, multimodal QP/fluorescent visualization of A549 biological cells fluorescently tagged for F-actin. Our results suggest that SI has a unique utility in studying biological phenomena with significant molecular, biophysical, and biochemical components.

DOI10.1364/BOE.8.002496
Short TitleBiomedical Optics Express